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Cluster analysis is one of the most common and useful tools in pattern recognition, statistical data analysis, and exploratory data mining. The main advantage of employing clustering techniques is the possibility of finding a hidden structure in the data without the requirement of prior information or knowledge about it. A clustering task consists of dividing a dataset into groups i. However, the K-means algorithm rely on the frequent computation of similarity metrics between all of the elements to be clustered and the proposed centroids of each of the k-clusters.
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Recent advances in the scale and diversity of population genomic datasets for bacteria now provide the potential for genome-wide patterns of co-evolution to be studied at the resolution of individual bases. Here we describe a new statistical method, genomeDCA, which uses recent advances in computational structural biology to identify the polymorphic loci under the strongest co-evolutionary pressures. We apply genomeDCA to two large population data sets representing the major human pathogens Streptococcus pneumoniae pneumococcus and Streptococcus pyogenes group A Streptococcus. For pneumococcus we identified 5, putative epistatic interactions between 1, sites. Over three-quarters of the links were between sites within the pbp2x , pbp1a and pbp2b genes, the sequences of which are critical in determining non-susceptibility to beta-lactam antibiotics.
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